PENGARUH CO2 TERHADAP LAJU PERTUMBUHAN DAN KANDUNGAN LIPID BOTRYOCOCCUS BRAUNII PADA MEDIA AIR LAUT

Verina J. Wargadalam, Edi Saadudin, Silvy R. Fitri

DOI: http://dx.doi.org/10.32693/jgk.13.1.2015.257

Abstract


Mikroalga sebagai salah satu opsi penyedia sumber bahan bakar nabati telah banyak diteliti. Salah satu yang perlu diperhatikan untuk keberlajutannya adalah sangat besarnya kebutuhan air yang harus disediakan dalam proses budidaya. Untuk menghindari kompetisi kebutuhan air tersebut, budidaya mikroalga dalam media air laut/salin dapat dipertimbangkan. Penelitian ini mempelajari pengaruh penambahan CO2 terhadap laju pertumbuhan dan kandungan lipid mikroalga Botryococcus braunii Kützing strain NIES-836 yang dibudidaya dalam media salin dengan sistem terbuka. Penambahan CO2 sebesar 2% hingga 20% meningkatkan laju pertumbuhan spesifik, dengan nilai tertinggi dicapai pada penambahan 2% CO2, yaitu 0,53/hari, dan laju pertumbuhan mulai menurun signifikan pada penambahan 20% CO2. Produksi lipid tertinggi yang dapat dipulihkan juga dicapai pada penambahan 2% CO2, yaitu sebesar 0,34 g/L. Kandungan asam lemak dalam total lipid diketahui hanya mencapai 25% dengan komposisi utamanya Metil Palmitat, Metil Palmitoleat dan Metil Oleat.

Kata kunci: Botryococcus braunii, CO2, laju pertumbuhan, lipid, air laut


Microalgae as an alternative resource for biofuel have attracted many research works, and one of the concerns is its sustainability due to the need of a huge amount of water in the cultivation process. To avoid such water competition, cultivation of microalgae in media of saline/sea water can be considered. In this works, the effects of CO2 on the growth and lipid accumulation of Botryococcus braunii Kützing strain NIES-836 in sea water/saline media and open system have been studied. The CO2 additions of 2% to 20% were found to increase the specific growth rate. The highest value was reached at 2% CO2 addition, i.e.: 0.53/day, whereas, the specific growth rate started to decrease significantly at 20% CO2 addition. The highest lipid production that could be recovered was 0.34 g/L, and that was observed at 2% CO2 addition. The fatty acid content in the total lipid was 25%, mainly consists of Methyl Palmitate, Methyl Palmitoleate, and Methyl Oleate.

Keywords: Botryococcus braunii, CO2, growth rate, lipid, sea water


Keywords


Botryococcus braunii;CO2;laju pertumbuhan;lipid;air laut

References


Ashokkumar, V., Rengasamy, R., 2012. Mass culture of Botryococcus braunii Kutz.under open raceway pond for biofuel production. Bioresource Technology. 104:394-399.

Banerjee A, Sharma, R., Chisti, Y., Banerjee, U.C. 2002. Botryococcus braunii: A Renewable Source of Hydrocarbons and Other Chemicals. Critical Reviews in Biotechnology. 22(3):245-279.

Carcano, S., 2010.A Model for Cell Growth in Batch Bioreactors. Disertasi, Facolta di Ingegneria dei Sistemi, Politecnico di Milano

Faridha, 2013.Pemanfaatan Emisi CO2 PLTU Batubara dan Air Limbah Domestik Yang Terintegrasi Untuk Pertumbuhan Mikroalga sebagai bahan Biodiesel dalm Mendukung Energi Berkelanjutan. Disertasi S3. Universitas Indonesia.

Ge, Y., Liu, J., Tian, G., 2011. Growth characteristics of Botryococcus braunii 765 under high CO2 concentration in photobioreactor. Bioresource Technology. 102(1):130-134.

Li, X., Hu, H., Gan ke, Sun, Y., 2010.Effects of different nitrogen and phosphorus concentrations on the growth, nutrient uptake, and lipid accumulation of a freshwater microalgae Scenedesmus sp. Bioresource Technology. 101(14): 5494-5500.

Metzger, P., Largeau, C., 2005. Botryococcus braunii: a rich source for hydrocarbons and related ether lipids. Applied Microbiology and Biotechnology. 66(5):486-496.

Ranga Rao, A., Dayananda, C., Sarada, R., Shamala, T.R., Ravishankar, G.A., 2007. Effect of Salinity on Growth of Green Alga Botryococcus braunii and its Constituents. Bioresource Technology. 98(3):560-564.

Saadudin, E., Fitri, S.R., Wargadalam, V.J., 2011. Karakteristik Asam Lemak Mikroalga Untuk Produksi Biodiesel. Ketenagalistrikan dan Energi Terbarukan. 10(2):131-140.

Senousy, H.H., Beakes, G.W., Hack, E. 2004. Phylogenetic Placement of Botryococcus braunii (Trebouxiophyceae) and Botryococcus Sudeticus Isolate Utex 2629 (Chlorophyceae). Journal of Phycology. 40:412-423.

Sydney, E.B., Sturm, W., Carvalho, J.C., Thomas-Soccol, V., Larroche, C., Pandley, Ashok, Soccol, C.R., 2010. Potential carbon dioxide fixation by industrially important microalgae. Bioresource Technology. 101(15):5892-5896.

Yamaguchi, K., Nakano, H., Murakami, M., Konosu, S., Nakayama, O., Nakamura, A., Iwamoto, H., 1987.Lipid Composition of green alga, Botryococcus braunii. Agriculture and Biological Chemistry. 51(2):493-498.

Yoshimura, T., Okada, S., Honda, M., 2013. Culture of the hydrocarbon producing microalga Botryococcus braunii strain Showa: Optimal CO2, salinity, temperature, and irradiance conditions. Bioresource Technology. 133: 232-239.




DOI: http://dx.doi.org/10.32693/jgk.13.1.2015.257

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